In vitro mRNA degradation system to study the virion host shutoff function of herpes simplex virus.

@article{Krikorian1991InVM,
  title={In vitro mRNA degradation system to study the virion host shutoff function of herpes simplex virus.},
  author={C R Krikorian and G. Sullivan Read},
  journal={Journal of virology},
  year={1991},
  volume={65 1},
  pages={112-22}
}
The virion host shutoff (vhs) gene of herpes simplex virus encodes a virion polypeptide that induces degradation of host mRNAs at early times and rapid turnover of viral mRNAs throughout infection. To better investigate the vhs function, an in vitro mRNA degradation system was developed, consisting of cytoplasmic extracts from HeLa cells infected with wild-type herpes simplex virus type 1 or a mutant encoding a defective vhs polypeptide. Host and viral mRNAs were degraded rapidly in extracts… CONTINUE READING

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