In vitro labeling of axonal projections in the mammalian central nervous system

  title={In vitro labeling of axonal projections in the mammalian central nervous system},
  author={Kerry L. Bernardo and Pokay M. Ma and Thomas A. Woolsey},
  journal={Journal of Neuroscience Methods},
HRP-filling of neurons and axonal arbors in fixed brain slabs
  • M. Freire
  • Biology
    Journal of Neuroscience Methods
  • 1990
Local axonal trajectories in mouse barrel cortex
The findings indicate that integration of information between whisker rows within barrel cortex involves asymmetrical connections within layer IV and infragranular layers.
Growth of thalamic afferents into mouse barrel cortex.
Thalamocortical afferent growth into somatosensory cortex as the whisker barrels emerge in postnatal mice is studied, finding the selection mechanism appears to result from within-group reinforcement events that are stronger for branches toward the center of each whisker-related Gaussian distribution.
Axonal trajectories between mouse somatosensory thalamus and cortex
An in vitro brain slice preparation has been used to label fibers connecting the somatosensory thalamus and cortex of the mouse with horseradish peroxidase crystals, HRP, nonidet P‐40 detergent chips, or a solution of HRP/dimethylsulfoxide.
A small subpopulation of progesterone receptor‐containing neurons in the guinea pig arcuate nucleus projects to the median eminence
In female guinea pigs, a combination of retrograde tracing and immunofluorescence for progesterone receptors (PR) was applied to determine if PR‐immunoreactive (PR‐IR) neurons in the arcuate nucleus
Local intra‐ and interlaminar connections in mouse barrel cortex
Focal injections of horseradish peroxidase in dimethylsulfoxide were targeted into mouse somatosensory cortex, in vitro, with a template to determine quantitative connectivity patterns within and between barrel‐defined cortical columns.
NADPH-Diaphorase Containing Neurons and Biocytin-labelled Axon Terminals in the Visual Cortex of Adult Rats Malnourished During Development.
The results indicate that the rat area 17 is affected differently by early malnutrition, regarding biocytin-labelled axon terminals, on the one hand, and NADPH-d-containing neurons, onThe other, which suggest that they are less sensitive to the injury represented byEarly malnutrition.
Morphology and Growth Patterns of Developing Thalamocortical Axons
It is increasingly evident that the actions of guidance factors depend critically on the cellular and molecular context in which they operate. For this reason we examined the growth cone morphology


Retrograde Axonal Transport in the Central Nervous System
This retrograde movement of protein from axon terminal to cell body suggests a possible mechanism by which neurons respond to their target areas.
The organization of thalamocortical relay neurons in the rat ventrobasal complex studied by the retrograde transport of horseradish peroxidase
The organization of thalamocortical relay neurons in the thalamic ventrobasal complex (VB) of the rat was investigated by the use of the retrograde axonal transport of horseradish peroxidase (HRP).
Uptake and retrograde axonal transport of peroxidase in hypoglossal neurons. Electron microscopical localization in the neuronal perikaryon.
A study was made on the subcellular localization of horseradish peroxidase in hypoglossal neurones following injection of this protein tracer into the tongue muscle of suckling mice. Our previous
The retrograde intraaxonal transport of horseradish peroxidase in the chick visual system: A light and electron microscopic study
Findings suport the concept of intraaxonal retrograde movement of HRP, which was found in organelles within the axons of the optic nerve but not in the extracellular spaces of the retina.
Morphology of central terminations of intra‐axonally stained, large, myelinated primary afferent fibers from facial skin in the rat
  • H. Hayashi
  • Biology
    The Journal of comparative neurology
  • 1985
The differential laminar distribution of collateral arbors of single axons along the rostrocaudal axis distinguishes the spinal trigeminal nucleus from the spinal dorsal horn where functional types of mechanoreceptive afferents form continuous or interrupted sagittal columns of terminal arbors that do not shift dorsoventrally within segments.
Intracellular injection with horseradish peroxidase of physiologically characterized stellate and bushy cells in slices of mouse anteroventral cochlear nucleus
  • S. WuD. Oertel
  • Biology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1984
Intracellular recordings from six stellate cells labeled by intracellular injection of HRP revealed that they have linear current-voltage relationships around the resting potential and that they respond to suprathreshold depolarization with large, regularly firing action potentials.
Spinal termination of functionally identified primary afferent neurons with slowly conducting myelinated fibers
Results suggest that there is a highly specialized central projection of primary afferent endings which is related to sensory function and not to fiber diameter, and which receives direct input from cutaneous receptors transmitting activity initiated by innocuous stimulation.