In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation

  title={In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation},
  author={Michael C. Jewett and Brian R. Fritz and Laura E. Timmerman and George M Church},
  booktitle={Molecular systems biology},
Purely in vitro ribosome synthesis could provide a critical step towards unraveling the systems biology of ribosome biogenesis, constructing minimal cells from defined components, and engineering ribosomes with new functions. Here, as an initial step towards this goal, we report a method for constructing Escherichia coli ribosomes in crude S150 E. coli extracts. While conventional methods for E. coli ribosome reconstitution are non-physiological, our approach attempts to mimic chemical… CONTINUE READING
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Reconstitution of ribosomes, in Ribosomes and Protein Synthesis, A Practical Approach

  • KH Nierhaus
  • 1990
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