In vitro infection of macrophages by HIV: correlation with cellular activation, synthesis of tumour necrosis factor alpha and proteolytic activity.

@article{Mabondzo1991InVI,
  title={In vitro infection of macrophages by HIV: correlation with cellular activation, synthesis of tumour necrosis factor alpha and proteolytic activity.},
  author={Alo{\"i}se M Mabondzo and Richard R Le Naour and Herv{\'e} Raoul and Pascal Clayette and Chantal Lafuma and F C Barr{\'e}-Sinoussi and Yvon E. Cayre and Dominique Dormont},
  journal={Research in virology},
  year={1991},
  volume={142 2-3},
  pages={205-12}
}
Macrophages were obtained after differentiation of healthy donor monocytes. Seven to 9 days after isolation, cells were infected with HIV1. Tumour necrosis factor alpha (TNF alpha) biological activity, TNF alpha- and 1-6-fructose-diphosphatase-gene expression and gelatinase activity were sequentially determined and correlated with viral infection and replication. TNF alpha was only detectable when mature viral particles were isolated in cell culture supernatants; 1-6-fructose diphosphatase mRNA… CONTINUE READING