In vitro induction of tetraploids in Arachis paraguariensis

@article{Aina2012InVI,
  title={In vitro induction of tetraploids in Arachis paraguariensis},
  author={Olubunmi Aina and Kenneth H. Quesenberry and M. D'Addabbo Gallo},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2012},
  volume={111},
  pages={231-238}
}
In this study, an efficient procedure was established for successful induction of tetraploid Arachis paraguariensis by treating diploid explants with colchicine. Quartered-seed, callus and shoot-tips were treated with colchicine at concentrations of 0.05, 0.1, 0.2 and 0.5 % (w/v) for 4, 8, 16, 20 and 24 h before they were transferred unto modified Murashige and Skoog medium for either callus induction or shoot regeneration. Results showed that quartered-seed displayed the highest frequency of… 
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