In vitro genotoxic effects of the anticancer drug gemcitabine in human lymphocytes.

@article{Aydemir2005InVG,
  title={In vitro genotoxic effects of the anticancer drug gemcitabine in human lymphocytes.},
  author={N. Aydemir and S. Çelikler and R. Bilaloğlu},
  journal={Mutation research},
  year={2005},
  volume={582 1-2},
  pages={
          35-41
        }
}
This research was carried out to investigate in vitro genotoxic effects of the anticancer agent gemcitabine on the induction of chromosomal aberrations and sister-chromatid exchange in human lymphocytes. Three doses of gemcitabine (0.001, 0.002 and 0.004 microg/ml) were applied to lymphocyte cultures from 15 donors. There was a significant increase in the induction of chromosome aberrations and in the occurrence of sister-chromatid exchange in these cells. In addition, gemcitabine significantly… Expand
Modulation of radiation-induced and mitomycin C-induced chromosome damage by apigenin in human lymphocytes in vitro
  • N. K. Sharma
  • Biology, Medicine
  • Journal of radiation research
  • 2013
TLDR
Investigation of the modulatory effects of APG on human lymphocytes after irradiation with gamma rays or treatment with the antineoplastic agent, mitomycin C, in vitro found no significant differences in the frequency of CAs or micronuclei in human peripheral blood lymphocytes irradiated with Gamma rays and then post-treated with APG. Expand
The Assessment of Cytotoxicity and Genotoxicity of Tetracycline Antibiotic in Human Blood Lymphocytes Using CBMN and SCE Analysis, in Vitro
Abstract Tetracycline antibiotic, a widely used antimicrobial drug was tested for nuclear DNA damage in cultured peripheral blood lymphocytes in terms of chromosome alterations. The extent ofExpand
Antigenotoxic effect of apigenin against anti-cancerous drugs.
TLDR
The treatment of apigenin results in a significant, dose dependent decrease in the genotoxic damage, induced by mitomycin C and cyclophosphamide, thereby reducing the chances of developing secondary tumors during the therapy. Expand
Antigenotoxic effect of lipoic acid against mitomycin-C in human lymphocyte cultures
TLDR
Results demonstrated anticlastogenic and antimutagenic effects of LA against MMC induced genotoxicity and are encouraging that LA can be a possible chemopreventive agent in tumorigenesis in both cancer patients and in health care persons handling anti-cancer drugs. Expand
Genotoxicity of all-trans retinoic acid (ATRA) and its steroidal analogue EA-4 in human lymphocytes and mouse cells in vitro.
TLDR
It is found that ATRA and its steroidal analogue EA-4 induce micronucleation mainly via chromosome breakage and chromosome delay in a lesser extent and generate multinucleated and multimicronucleated interphase cells. Expand
Genotoxic evaluation of terbinafine in human lymphocytes in vitro
TLDR
Results of this study showed that terbinafine neither induced CA, SCE, MN, NP and NB nor affected significantly mitotic, replication and cytokinesis-block proliferation indices in any of the tested concentrations. Expand
Cytotoxicity of neoplastic drugs Gefitinib, Cisplatin, 5-FU, Gemcitabine, and Vinorelbine on human cervical cancer cells (HeLa)
TLDR
The aim of this investigation was to determine the chemo sensitivity of the five commonly used neoplastic drugs such as Geftinib, Cisplatin, 5-FU, Gemcitabine and Vinorelbine in vitro, and compare its toxicity on cervical cancer cells (HeLa) using lymphocytes (nucleated cells) as controls. Expand
Anti-mutagenic activity of Salvia merjamie extract against gemcitabine.
  • K. Alanazi
  • Biology, Medicine
  • Asian Pacific journal of cancer prevention : APJCP
  • 2015
TLDR
Results demonstrate that Salvia merjamie extract protects bone marrow cells in mice against gemcitabine induced mutagenicity, which can be used for the development of a potential therapeutic anti-mutagenic agents. Expand
Toxicogenomic activity of gemcitabine in two TP53-mutated bladder cancer cell lines: special focus on cell cycle-related genes
TLDR
Gemcitabine had distinct toxicogenomic effects in the bladder transitional carcinoma cell lines with two different TP53 mutations, however, independent of the type of mutation and tumor grade, gem citabine induced cell cycle arrest; upregulation of DNA repair-related genes, G1/S transition, apoptosis and activation of transcription factors, mainly by up regulation of the CCNE1, CDKN1A and GADD45A genes. Expand
Antigenotoxic effect of apigenin against mitomycin C induced genotoxic damage in mice bone marrow cells.
  • Y. Siddique, Mohammad Afzal
  • Biology, Medicine
  • Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2009
TLDR
A significant decrease in SCEs and CAs was observed, suggesting a protective role of apigenin against the genotoxicity of mitomycin C on mice bone marrow cells. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 21 REFERENCES
Genotoxicity of two anticancer drugs, gemcitabine and topotecan, in mouse bone marrow in vivo.
TLDR
The results of this study indicate that the two chemotherapeutics gemcitabine and topotecan have cytotoxic and genotoxic effects in mouse bone marrow. Expand
Gemcitabine cytotoxicity of human malignant glioma cells: modulation by antioxidants, BCL-2 and dexamethasone.
TLDR
Gemcitabine induces cytotoxic and clonogenic death of 12 human malignant glioma cell lines at clinically relevant concentrations around 1 microM and is thus approximately 100-fold more active than the congener drug, cytarabine. Expand
Effects of gemcitabine and araC on in vitro DNA synthesis mediated by the human breast cell DNA synthesome
TLDR
dC is a more potent inhibitor of intact cell DNA synthesis and in vitro SV40 DNA replication than araC and the MCF7 cell DNA synthesome can serve as a unique and relevant model to study the mechanism of action of anticancer drugs that directly affect DNA synthesis. Expand
Characterisation of genotoxic properties of 2',2'-difluorodeoxycytidine.
TLDR
The genotoxic properties of 2',2'-difluorodeoxycytidine (dFdC) were characterised using diploid, mortal low-passage fibroblasts (LPF cells) and the spontaneously transformed fibroblast cell line V79, and chromosomal alterations were detected by the micronucleus assay within a narrow concentration range. Expand
Induction of apoptosis by gemcitabine.
TLDR
The generation of large-sized DNA fragments caused by incorporated gem citabine monophosphate in DNA is critical in gemcitabine-induced apoptosis, whereas nucleosomal DNA fragmentation is not a requirement in this cell death process. Expand
Supraadditive effect of 2′,2′-difluorodeoxycytidine (gemcitabine) in combination with oxaliplatin in human cancer cell lines
TLDR
The data show that the combination of gemcitabine with oxaliplatin exerts potent antiproliferative effects in human leukemia and colon cancer cells, warranting further investigations in the framework of phase I–II trials as an alternative for the treatment of solid malignancies. Expand
Chromosome aberrations, micronuclei, aneuploidy, sister chromatid exchanges, and cancer risk assessment.
TLDR
The aim of this paper is to encourage cytogeneticists to design their experiments in such a way that the data obtained will be of maximum possible benefit for characterizing and quantifying adverse human health effects, particularly cancer. Expand
Cytogenetic consequences after occupational exposure to antineoplastic drugs.
TLDR
The results of this study show that biomonitoring after exposure to a mixture of antineoplastic drugs which express clastogenic and aneugenic activity should involve a battery of cytogenetic methods. Expand
Difluorodeoxyguanosine: cytotoxicity, metabolism, and actions on DNA synthesis in human leukemia cells.
TLDR
Observations indicated that dGuo kinase, which phosphorylates arabinosylguanine, also appears to play a major role in activating dFdG, which is a promising new antimetabolite. Expand
Combination therapy with gemcitabine in non-small cell lung cancer.
TLDR
Preliminary results must be validated by large randomised trials comparing gemcitabine-containing regimens with NSCLC reference chemotherapy regimens, which showed a favourable safety-efficacy profile and compared well with standard regimens used inNSCLC. Expand
...
1
2
3
...