In vitro generation of type-II pneumocytes can be initiated in human CD34+ stem cells

  title={In vitro generation of type-II pneumocytes can be initiated in human CD34+ stem cells},
  author={Lokanathan Srikanth and Katari Venkatesh and Manne Mudhu Sunitha and Pasupuleti Santhosh Kumar and C. Chandrasekhar and Bhuma Vengamma and Potukuchi Venkata Gurunadha Krishna Sarma},
  journal={Biotechnology Letters},
ObjectivesHuman CD34+ stem cells differentiated into type-II pneumocytes in Dulbecco’s modified Eagle medium (DMEM) having hydrocortisone, insulin, fibroblast growth factor (FGF), epidermal growth factor (EGF) and bovine serum albumin (BSA), expressing surfactant proteins-B (SP-B) and C (SP-C), alkaline phosphatase (ALP) and lysozyme.ResultsFACS-enumerated pure CD34+ cells, isolated from human peripheral blood, were cultured in DMEM and showed positive reaction with anti-human CD34 monoclonal… 
In vitro differentiation potential of human haematopoietic CD34+ cells towards pancreatic β‐cells
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Gel based in vitro 3D model exploring the osteocytic potentiality of human CD34+ stem cells
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Down-regulation of PAX2 promotes in vitro differentiation of podocytes from human CD34+ cells
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Initial observations on the effect of medium composition on the differentiation of murine embryonic stem cells to alveolar type II cells.
Observations in this study represent an initial step towards achieving directed differentiation of pneumocytes from stem cells that could lead to their purification for tissue engineering purposes.
In vitro cardiogenesis can be initiated in human CD34+ cells.
It is observed that the transplantation of autologous stem cells/fetal cardiomyocytes in the heart scar tissue developed due to infarct, limited the scar expansion, and prevented post infarCT heart failures.
Pulmonary surfactant secretion in briefly cultured mouse type II cells.
  • L. Gobran, S. Rooney
  • Biology
    American journal of physiology. Lung cellular and molecular physiology
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The data establish that briefly cultured type II cells provide a suitable model for investigation of surfactant secretion in normal and genetically altered mice.
Alkaline phosphatase: a marker of alveolar type II cell differentiation.
In the adult rat alveolus, alkaline phosphatase staining selectively identified type II cells, although nonciliated bronchiolar (Clara) cells and loose perivascular connective tissue also stained for alkalineosphatase activity.
Effects of intralipid and hydrocortisone upon human fetal lung cell cultures
The application of Intralipid represents an alternative method to accelerate antenatal surfactant production and to improve the rate of survival of preterm infants.
The hypothesis that MLE-15 cells grown within the microfiber culture system in the presence of airflow maintain the phenotypic characteristics of type II cells to a higher degree than those grown in standard in vitro cell culture models is supported.
Targeted disruption of the surfactant protein B gene disrupts surfactant homeostasis, causing respiratory failure in newborn mice.
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    Proceedings of the National Academy of Sciences of the United States of America
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The role of SP-B in lung function was assessed by disrupted by homologous recombination in murine mouse embryonic stem cells and an aberrant form of pro-SP-C, 8.5 kDa, was detected, and fully processed SP-C peptide was markedly decreased in lung homogenates of SP -/- mice.
Plasticity of bone marrow-derived stem cells
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It is shown that beta-2-microglobulin and peptidyl-prolylisomerase A were the optimal reference genes for normalizing RT-qPCR data obtained from MSC, whereas the TATA box binding protein was not suitable due to its extensive variability in expression.