In vitro generation of type-II pneumocytes can be initiated in human CD34+ stem cells

  title={In vitro generation of type-II pneumocytes can be initiated in human CD34+ stem cells},
  author={L. Srikanth and K. Venkatesh and M. M. Sunitha and Pasupuleti Santhosh Kumar and C. Chandrasekhar and B. Vengamma and P. Sarma},
  journal={Biotechnology Letters},
ObjectivesHuman CD34+ stem cells differentiated into type-II pneumocytes in Dulbecco’s modified Eagle medium (DMEM) having hydrocortisone, insulin, fibroblast growth factor (FGF), epidermal growth factor (EGF) and bovine serum albumin (BSA), expressing surfactant proteins-B (SP-B) and C (SP-C), alkaline phosphatase (ALP) and lysozyme.ResultsFACS-enumerated pure CD34+ cells, isolated from human peripheral blood, were cultured in DMEM and showed positive reaction with anti-human CD34 monoclonal… Expand
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  • L. Gobran, S. Rooney
  • Biology, Medicine
  • American journal of physiology. Lung cellular and molecular physiology
  • 2004
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