In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes.

@article{Janse1985InVF,
  title={In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes.},
  author={Chris J Janse and Barend Mons and R. J. Rouwenhorst and P F van der Klooster and J P Overdulve and H J van der Kaay},
  journal={Parasitology},
  year={1985},
  volume={91 ( Pt 1)},
  pages={19-29}
}
In vitro formation of Plasmodium berghei ookinetes was studied. Gametocytes produced in vitro were obtained from heart and tail blood of Swiss mice and from blood removed from mosquitoes directly after feeding on these mice. In vitro produced gametocytes were obtained from short-term cultures of the erythrocytic stages of P. berghei. Reproducible ookinete production was obtained in medium RPMI 1640, pH 7.8-8.0, using in vivo and in vitro produced gametocytes. The morphology of developmental… CONTINUE READING