• Published 2017

In vitro fertilizations with cryopreserved sperm of Rhinella marina ( Anura : Bufonidae ) in Ecuador

@inproceedings{Proao2017InVF,
  title={In vitro fertilizations with cryopreserved sperm of Rhinella marina ( Anura : Bufonidae ) in Ecuador},
  author={Bel{\'e}n Proa{\~n}o and Oscar Dold{\'a}n P{\'e}rez},
  year={2017}
}
—Considering worldwide amphibian population decline, sperm cryopreservation should be a priority for conservation of species in areas of high biodiversity, such as the Neotropics. In this study, we present the results of two cryopreservation experiments involving Rhinella marina sperm. Freezing was performed in a -80 °C freezer and dimethyl sulfoxide (DMSO) was used as cryoprotective agent. In the first experiment, the effects of 5%, 10%, and 15% DMSO were evaluated in sperm lysis and… CONTINUE READING

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