In vitro fertilization of horse follicular oocytes matured in vitro.

@article{Zhang1990InVF,
  title={In vitro fertilization of horse follicular oocytes matured in vitro.},
  author={Jian Ju Zhang and L Z Muzs and M S Boyle},
  journal={Molecular reproduction and development},
  year={1990},
  volume={26 4},
  pages={361-5}
}
In vitro fertilizing ability of stallion spermatozoa was assessed using horse follicular oocytes matured in vitro. After collection, stallion spermatozoa were either: 1) washed and incubated in TALP medium with 3 mg/ml bovine serum albumin (BSA) and 10 micrograms/ml heparin for 4h, 2) washed and incubated in TALP with 3 mg/ml BSA for 3 h and cultured for a further 1 h with 1 mM caffeine and 5 mM dbcAMP, 3) washed and incubated in TALP medium with 3 mg/ml BSA at pH 7.9-8.2 for 2-4 h, or 4… CONTINUE READING