In vitro fertilization and development of immature and mature bovine oocytes cryopreserved by ethylene glycol with sucrose.


Immature and mature bovine oocytes were frozen slowly in 1.8 M ethylene glycol (EG) with different concentrations of sucrose (0, 0.1, and 0.2 M). After thawing and dilution of the cryoprotectant by a one-step procedure, the survival of the oocytes was assessed morphologically and also by in vitro fertilization and culture. Sucrose had no effect on the… (More)


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