In vitro expansion of human peripheral blood CD34+ cells.

@article{Sato1993InVE,
  title={In vitro expansion of human peripheral blood CD34+ cells.},
  author={Naoaki Sato and Kenichi Sawada and Kazuki Koizumi and Takashi Tarumi and Masahiro Ieko and Takanori Yasukouchi and Miki Yamaguchi and Tsuneo A. Takahashi and Sadayoshi Sekiguchi and Tadashi Koike},
  journal={Blood},
  year={1993},
  volume={82 12},
  pages={3600-9}
}
To elucidate the role of recombinant human colony-stimulating factors (CSFs) for expanding peripheral blood (PB) CD34+ cells, these cells were purified up to 94.5% +/- 1.3% and the effects of individual and combined CSFs on the proliferation and differentiation of these cells were studied in a 7-day suspension culture. The majority of CD34+ cells coexpressed CD38 (81.8% +/- 5.1%), but was negative for CD33 (88.5% +/- 3.4%). Among the individual CSFs examined, recombinant interleukin-3 (rIL-3… CONTINUE READING
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