In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells.

@article{Spencer2012InVE,
  title={In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells.},
  author={Nakia D. Spencer and Raymond Y. T. Chun and Martin A. Vidal and Jeffrey M. Gimble and Mandi J. Lopez},
  journal={Veterinary journal},
  year={2012},
  volume={191 2},
  pages={
          231-9
        }
}
The objective of this study was to determine the tissue density, in vitro expansion and differentiation of canine adipose tissue-derived (ASC) and bone marrow-derived (BMSC) stromal cells. Primary (P0) and cell passages 1-6 (P1-6) cell doubling numbers (CD) and doubling times (DT) were determined in fresh cells. The P0, P3, and P6 adipogenic (CFU-Ad), osteogenic (CFU-Ob), and fibroblastic (CFU-F) colony forming unit frequencies, lineage specific mRNA levels in differentiated P3 cells and… CONTINUE READING

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