In vitro evolution of a neutralizing human antibody to human immunodeficiency virus type 1 to enhance affinity and broaden strain cross-reactivity.

@article{Barbas1994InVE,
  title={In vitro evolution of a neutralizing human antibody to human immunodeficiency virus type 1 to enhance affinity and broaden strain cross-reactivity.},
  author={Carlos F. Barbas and Dong-xu Hu and Nicola Dunlop and Lynette Sawyer and D Cababa and R. Michael Hendry and Peter L. Nara and Dennis R. Burton},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 9},
  pages={3809-13}
}
A method is described that allows for the improvement of antibody affinity. This method, termed complementary-determining region (CDR) walking, does not require structural information on either antibody or antigen. Complementary-determining regions are targeted for random mutagenesis followed by selection for fitness, in this case increased binding affinity… CONTINUE READING