In vitro embryo production in llamas (Lama glama) from in vivo matured oocytes with raw semen processed with Androcoll-E using defined embryo culture media.

@article{Trasorras2012InVE,
  title={In vitro embryo production in llamas (Lama glama) from in vivo matured oocytes with raw semen processed with Androcoll-E using defined embryo culture media.},
  author={Virginia Luz Trasorras and S. Giuliano and Graciela Chaves and Deborah M. Neild and Alicia Ag{\"u}ero and Manuel Carretero and Manuel Pinto and C Baca Castex and Angel Alonso and Daniel Rodriguez and Jane Margaret Morrell and M Marrero Miragaya},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2012},
  volume={47 4},
  pages={562-7}
}
The aim of this study was to carry out in vitro fertilization using spermatozoa selected with Androcoll-E™ and to evaluate the efficiency of the culture medium DMEM-F12 for in vitro embryo development in the llama. Twelve adult females from 18 superstimulated (67%) were used as oocyte donors. They were superstimulated with 1500 IU of eCG and after 5 days, received a single dose of buserelin. Twenty hours post-injection, follicular aspiration was conducted by flank laparotomy. Semen collections… CONTINUE READING

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