In vitro effects of lactoferrin on lipopolysaccharide-induced proliferation, gene expression, and prostanoid production by bovine peripheral blood mononuclear cells.

@article{Dawes2008InVE,
  title={In vitro effects of lactoferrin on lipopolysaccharide-induced proliferation, gene expression, and prostanoid production by bovine peripheral blood mononuclear cells.},
  author={Maisie E. Dawes and Jeff W. Tyler and Antoinette E. Marsh and Robert L. Larson and Barry J Steevens and Jeffrey Lakritz},
  journal={American journal of veterinary research},
  year={2008},
  volume={69 9},
  pages={1164-70}
}
OBJECTIVE To evaluate the effect of lactoferrin on lipopolysaccharide (LPS)-induced proliferation of bovine peripheral blood mononuclear cells (PBMCs), gene expression of inflammatory mediators, and production of prostanoids in vitro. SAMPLE POPULATION PBMCs isolated from 15 Holstein bull calves. PROCEDURES Mixed populations of PBMCs were isolated by differential centrifugation. Proliferation assays were conducted in 96-well plates designed to allow addition of lactoferrin (200 ng/mL) with… CONTINUE READING

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