In vitro effect of neuropeptide Y on melatonin and norepinephrine release in rat pineal gland

1. To study neuropeptide Y (NPY) effect on melatonin production, rat pineal explants were incubated for 6 hr with 10–1000 nM NPY in the presence or absence of 10µM norepinephrine (NE). Melatonin content in the pineal gland and media was measured by radioimmunoassay (RIA). 2. NPY (10–1000 nM) increased melatonin production and, at 10 or 100 nM concentrations… CONTINUE READING