In vitro dissociation and reassociation of the symbionts of the lichenHeppia echinulata

Heppia echinulata, a heteromerous, cyanophilic lichen has been cultured on a nutrient medium containing silica gel powder. The paper describes the formation of new thalli by dissociation of the components, reversion to their free-living form and complete resynthesis accomplished as a continuous process under the same culture conditions.