In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection.

@article{Liang2011InVD,
  title={In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection.},
  author={Yuanyuan Liang and Tatsanee Phermthai and Takashi Nagai and T Somfai and Rangsun Parnpai},
  journal={Theriogenology},
  year={2011},
  volume={75 9},
  pages={1652-60}
}
The objective of this study was to investigate the potential of swamp buffalo oocytes vitrified-warmed at the metaphase of the second meiotic cell division (M-II) stage to develop to the blastocyst stage after parthenogenetic activation (PA) or intracytoplasmic sperm injection (ICSI). In Experiment 1, we examined the effects of exposure time of oocytes to cryoprotectants (CPA) on their in vitro development after PA. In vitro matured (IVM) oocytes were placed in 10% dimethylsulfoxide (DMSO) + 10… CONTINUE READING