In-vitro development of refrozen mouse embryos.

  title={In-vitro development of refrozen mouse embryos.},
  author={N J Vitale and Myrth Myers and Richard S. Denniston and Stanley P. Leibo and Robert A. Godke},
  journal={Human reproduction},
  volume={12 2},
To evaluate the effects of sequential, repetitive freezing on their in-vitro development, mouse embryos at the eight- to 16-cell stage were subjected to one of five treatments. They were (i) cultured as unfrozen controls, (ii) frozen once and cultured, (iii) subjected to two consecutive freeze-thaw cycles, (iv) frozen and thawed, and then cultured for 18-30 h before being frozen a second time, and (v) frozen three times in succession without being cultured. To assess their functional survival… CONTINUE READING

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