In vitro development of non-enucleated rat oocytes following microinjection of a cumulus nucleus and chemical activation.

@article{Fujii2008InVD,
  title={In vitro development of non-enucleated rat oocytes following microinjection of a cumulus nucleus and chemical activation.},
  author={Wataru Fujii and H Funahashi},
  journal={Zygote},
  year={2008},
  volume={16 2},
  pages={117-25}
}
The present study examined in vitro development and the cytological status of non-enucleated rat oocytes after microinjection of cumulus nuclei and chemical activation. Oocyte-cumulus complexes were collected from gonadotropin-treated prepubertal female Wistar rats 14 h after human chorionic gonadotropin (hCG) injection. Cumulus nuclei were injected into ovulated oocytes and then stimulated in the presence of 5 mM SrCl2 for 20 min at various time points (0-3.5 h) after injection. Some of the… CONTINUE READING
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