In vitro development of mouse pronuclear embryos to blastocysts in sequential media with and without co-culture of autologous cumulus cells.

@article{Farouk2008InVD,
  title={In vitro development of mouse pronuclear embryos to blastocysts in sequential media with and without co-culture of autologous cumulus cells.},
  author={Farha Naomi Omar Farouk and Marcela Vlad},
  journal={The Journal of reproduction and development},
  year={2008},
  volume={54 5},
  pages={
          385-90
        }
}
The objective of this study was to use mouse embryos as a model system to investigate the effect of co-culture of cumulus cells in Sydney IVF sequential media (Cook) on embryo development, based on the hypothesis that feeder cells in co-culture with a sequential medium could work synergistically to further improve in vitro culture conditions for mammalian preimplantation embryos. The culture systems described here were evaluated by the ability to consistently produce high blastocyst formation… CONTINUE READING
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