In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence.

@article{Gerger2010InVD,
  title={In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence.},
  author={R. R. Gerger and Eduardo S Ribeiro and Fabiana Forell and Luciana Relly Bertolini and Jos{\'e} Guilherme Lança Rodrigues and Carlos E Ambr{\'o}sio and M A Miglino and A. Lorenzo Mezzalira and Marcelo Bertolini},
  journal={Genetics and molecular research : GMR},
  year={2010},
  volume={9 1},
  pages={
          295-302
        }
}
The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence levels on in vitro development of cloned bovine embryos. In vitro-matured bovine oocytes were manually bisected and selected by DNA staining. One or two enucleated hemi-cytoplasts were paired and fused with an adult skin somatic cell. Cultured skin cells from an adult Nellore… CONTINUE READING

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