In vitro development following one-step dilution of OPS-vitrified porcine blastocysts.

@article{Cuello2004InVD,
  title={In vitro development following one-step dilution of OPS-vitrified porcine blastocysts.},
  author={Cristina Cuello and Maria A Gil and Inmaculada Parrilla and Jose Tornel and Juan Mar{\'i}a V{\'a}zquez and Jordi Roca and Françoise Berthelot and Françoise Martinat-Bott{\'e} and Emilio Martinez},
  journal={Theriogenology},
  year={2004},
  volume={62 6},
  pages={1144-52}
}
The objective of this experiment was to compare the in vitro survival and hatching rates of OPS-vitrified porcine blastocysts obtained after conventional (three-step dilution) or direct (one-step dilution) warming procedures. Expanded blastocysts were collected by laparotomy from weaned crossbred sows (n=7) on Day 6 of the cycle (D0: onset of estrus). Vitrification was performed as described by Berthelot et al. [Cryobiology 41 (2000) 116] using 17% (v/v) ethylene glycol and 17% (v/v) dimethyl… CONTINUE READING

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