In vitro development and characterization of a manatee bronchial cell line

  title={In vitro development and characterization of a manatee bronchial cell line},
  author={James M. Sweat and Calvin M. Johnson and Edward Paul Johnson Gibbs},
  journal={In Vitro Cellular & Developmental Biology - Animal},
In the present study, culture conditions that promote the growth and differentiation of manatee respiratory tract epithelial cells toward a mucociliary phenotype were determined. Characterization of a manatee-specific cell line enables investigators to conduct in vitro testing where live-animal experimentation is not possible. Cell cultures were established from both explants and enzymatically dissociated cells that were isolated from manatee bronchial tissue. To modulate their differentiation… CONTINUE READING


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