In vitro determination of tritiated thymidine labeling index (LI). Evaluation of a method utilizing hyperbaric oxygen and observations on the LI of human mammary carcinoma.


A simple method for incubation of tissue specimens with tritiated thymidine under 3 atm oxygen tension gave an in vitro labeling index (LI) that corresponded closely with LI's determined by injection of tritiated thymidine in vivo. The LI of tumors was not affected by storage at room temperature for up to 135 minutes. The mean LI for 39 human mammary… (More)


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