In vitro definition of the yeast RNA polymerase I enhancer.

  title={In vitro definition of the yeast RNA polymerase I enhancer.},
  author={Michael Schultz and Soo Young Choe and Ronald H. Reeder},
  journal={Molecular and cellular biology},
  volume={13 5},
In vitro conditions are reported under which an EcoRI-HpaI fragment of the Saccharomyces cerevisiae ribosomal gene spacer will enhance transcription from an adjacent RNA polymerase I promoter. Enhancement is largely independent of orientation and distance and is proportional to copy number. Mapping experiments reveal that two separate regions of the EcoRI-HpaI fragment are independently capable of promoter stimulation. These regions appear to correspond to elements which have been shown by… CONTINUE READING