In vitro culture promotes partial autonomous endosperm development in unfertilized ovules of wild-type Arabidopsis thaliana var. Columbia

@article{Rojek2005InVC,
  title={In vitro culture promotes partial autonomous endosperm development in unfertilized ovules of wild-type Arabidopsis thaliana var. Columbia},
  author={Joanna Rojek and Elżbieta Kuta and Jerzy Bohdanowicz},
  journal={Sexual Plant Reproduction},
  year={2005},
  volume={18},
  pages={29-36}
}
Partial endosperm development without paternal genome involvement was induced in unpollinated ovaries of wild-type Arabidopsis thaliana cultured in vitro. Unpollinated pistils were cultured on hormone-free Murashige and Skoog (MS) medium with addition of 6% sucrose and supplemented with: benzylaminopurine (BAP; 2 mg l−1) combined with naphthylacetic acid (NAA; 0.1 mg l−1), 2,4-dichlorophenoxyacetic acid (2,4-D; explants exposed to 1-h auxin shock 20 or 40 mg l−1, and transferred to hormone-free… CONTINUE READING

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