In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium

@article{Peng2010InVC,
  title={In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium},
  author={Xiayu Peng and Mei Yang and Liqin Wang and Chen Tong and Zhiqin Guo},
  journal={Journal of Assisted Reproduction and Genetics},
  year={2010},
  volume={27},
  pages={247-257}
}
This study evaluates the effect of a sequential culture system on the follicular development of sheep lamb ovaries, aiming to establish an available in vitro culture system for ovarian culture. Lamb ovarian cortical fragments were cultured on a steel mesh with a nitrocellulose membrane pre-coated by type1 collagen. Several culture media were used for the determinations, specifically, a control medium (α-MEM), a constant medium (control medium supplemented with 75 ng/mL human recombinant EGF… CONTINUE READING

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