In vitro culture of ovarian follicles.

  title={In vitro culture of ovarian follicles.},
  author={Geraldine Hartshorne},
  journal={Reviews of reproduction},
  volume={2 2},
This review offers a practically oriented introduction to follicle culture in vitro, focusing on mouse follicles, but with reference to other species. The main principles of follicle growth are addressed, including the constraints of tissue culture, methods of follicle isolation, and techniques for individual and collective culture of intact follicles. Culture systems that support a spherical or a non-spherical follicular structure in vitro are discussed in terms of follicular and oocyte… CONTINUE READING

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