In vitro culture of hymenopterous larval endoparasitoids

@article{Greany1986InVC,
  title={In vitro culture of hymenopterous larval endoparasitoids},
  author={P. Greany},
  journal={Journal of Insect Physiology},
  year={1986},
  volume={32},
  pages={409-419}
}
  • P. Greany
  • Published 1986
  • Biology
  • Journal of Insect Physiology
Abstract The solitary endoparasitoids Cotesia (= Apanteles) marginiventris and Microplitis croceipes, which attack noctuid larvae, were successfully cultured from post-germband stage eggs to fully grown first-instar larvae in completely artificial (but undefined) media. However, growth rates were slower than in vivo, and while some larvae of each species attained apparent competence to moult (based upon characteristic size and form), none did so. Up to 75% of pregermband M. croceipes eggs… Expand
In vitro development ofCampoletis sonorensis (Hym.: Ichneumonidae), a larval endoparasitoid ofheliothis virescens (Lep.: Noctuidae) in an artificial medium with insect sources from egg to third larval instar
TLDR
Host pupal hemolymph, fat body, and epidermal cell extracts on growth and development of Campoletis sonorensis in vitro were studied and it was found that these host-derived growth factors increased molting and growth but failed to stimulate C. sonoresis to develop to stages beyond the third instar. Expand
Embryonic development of an endoparasitoid,Microplitis croceipes (Hymenoptera: Braconidae) in cell line-conditioned media
TLDR
The composition of the medium and the species and tissue type of the cell line source must be evaluated interactively to determine optimal conditions for promoting development of M. croceipes in vitro. Expand
In vitro rearing of Toxoneuron nigriceps (Hymenoptera: Braconidae), a larval endoparasitoid of Heliothis virescens (Lepidoptera: Noctuidae) from early second instar to third instar larvae.
TLDR
Some of the in vitro reared third instar larvae demonstrated behavioral changes that could be interpreted as the preparation for cocoon formation or pupation i.e. oral secretion of a whitish material and lots of twisting and turning; however, none produced a cocoon nor pupa. Expand
In vitro rearing ofLysiphlebus fabarum [Hym.: Braconidae]
TLDR
In vitro rearing of the aphid endoparasitoid Lysiphlebus fabarum (Marshall) (Hymenoptera, Braconidae) was attempted, but only 2 out of the 48 tested in the 3 substrates became adults. Expand
Caste formation in the polyembryonic wasp Copidosoma floridanum (Hymenoptera: Encyrtidae): in vivo and in vitro analysis.
TLDR
Results indicate that embryos forming precocious and reproductive larvae acquire the competence to undergo morphogenesis at different times, but there is no evidence to suggest that caste determination is mediated by environmental factors associated with a specific stage of the host. Expand
In vitro rearing of Campoletis sonorensis, a larval endoparasitoid of Heliothis virescens from egg to third instar in an artificial medium devoid of insect sources
TLDR
Trehalose, lysine, threonine, asparagine, glutamine, hydroxyproline, serine, bovine serum albumin, and lactalbumin were beneficial to C. sonorensis in vitro and addition of fresh chicken egg yolk at a low level improved the artificial media. Expand
Microplitis croceipes teratocytes cause developmental arrest of Heliothis virescens larvae
TLDR
Treatment with hemocyte-and teratocyte-free hemolymph from parasitized larvae, hemocytes from nonparasitized H. virescens, unfertilized M. croceipes eggs, Cotesia congregata teratocytes, or Micrococcus lysodeikticus cells all had very little effect either on larval growth or development time. Expand
Development and encapsulation of the endoparasitoid,Microplitis croceipes (Hym.: Braconidae), in six candidate host species (Lep.)
TLDR
This biological information will be useful in additional laboratory studies directed toward reducing the rate of encapsulation of the endoparasitoid, Microplitis croceipes, in atypical lepidopteran host species whose usual host isHelicoverpa zea (Boddie). Expand
In vitro culture of the egg parasitoid Telenomus heliothidis: the role of teratocytes and medium consumption in development
TLDR
The solitary egg parasitoid Telenomus heliothidis Ashmead was reared from egg to adult in vitro and both sexes of adults emerged, mated, and the females produced viable offspring in the natural host. Expand
In vitro rearing of Exorista larvarum on tissue culture‐based diets
TLDR
The results demonstrate that E. larvarum is the most promising parasitoid ever studied for in vitro mass production. Expand
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Trichogramma pretiosum Riley, an obligate endoparasitic wasp of lepidopteran eggs, is the first insect egg parasite reared from egg to adult in vitro . It was reared on a medium devoid of host eggExpand
Artificial culture of the insect parasite, Pachycrepoideus vindemiae
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The parasite completed its larval development in the absence of host extracts on chemically defined media containing free-amino acids, fatty acids, cholesterol, inorganic salts, glucose and vitamins, and further development, however, was not obtained. Expand
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Abstract Changes in haemolymph proteins of the fall armyworm, Spodoptera frugiperda , associated with parasitism by the parasitoid Cotesia (= Apanteles) marginiventris were monitored by sodiumExpand
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During investigations on egg development in Pimpla1–5 the question arose as to what actually causes the conclusion of meiosis and initiation of cleavage in the oocyte nucleus after oviposition, and also what induces the beginning of the characteristic morphogenetic movements of the ooplasm in initial regions5. Expand
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TLDR
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TLDR
The role of virus-like particles in the calyx is discussed in the suppression of the host's immune response when H. zea is parasitized by the braconid, Microplitis croceipes, or the ichneumonid, Campoletis sonorensis, and the immune response of the hosts towards C. nigriceps is suppressed. Expand
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TLDR
In appropriate experiments, ecdysone was found to have no obvious effect on cultures of naked cysts and was neither able to replace nor enhance the activity of MF in the in vitro assay, which is present in blood plasma of both male and female pupae. Expand
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