In vitro culture of Cucumis sativus L. XVIII. Plants from protoplasts through direct somatic embryogenesis

@article{Burza2004InVC,
  title={In vitro culture of Cucumis sativus L. XVIII. Plants from protoplasts through direct somatic embryogenesis},
  author={Wojciech Burza and Stefan Malepszy},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={41},
  pages={259-266}
}
A procedure is described for the isolation and culture of protoplasts from embryogenic callus (gel-like callus — GLC) and embryogenic suspension cultures (ESC) of Cucumis sativus c.v. Borszczagowski. Maximal protoplast yields from GLC and ESC were 5×106 and 1×107 protoplasts/g tissue respectively. They were obtained following 14–16 h digestion with 1.2% Cellulase Onozuka R-10, 1.2% Macerozyme R-10 and 0.3% Driselase. At a plating density of 2×105 / ml, first divisions occurred in 4–5 days and 7… CONTINUE READING