In vitro culture and differentiation of buffalo (Bubalus bubalis) spermatogonia.

  title={In vitro culture and differentiation of buffalo (Bubalus bubalis) spermatogonia.},
  author={Bing-kun Xie and Zi-shun Qin and Ben Huang and T Xie and Hai-dong Yao and Yunwei Wei and Xianmei Yang and Deshun Shi and Heng Jiang},
  journal={Reproduction in domestic animals = Zuchthygiene},
  volume={45 2},
The objective of this study was to develop a culture system which could support buffalo spermatogonia differentiation into spermatids in vitro. Testes from 3- to 5-month-old buffaloes were decapsulated and seminiferous tubules were enzymatically dissociated to recover spermatogonia and sertoli cells. The cells were cultured in modified Dulbecco modified Eagle medium supplemented with different concentrations of foetal bovine serum, retinol, testosterone for 2 months at 37 degrees C… CONTINUE READING
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