In vitro culture and cryopreservation of Uronema marinum isolated from farmed New Zealand groper (Polyprion oxygeneios).

  title={In vitro culture and cryopreservation of Uronema marinum isolated from farmed New Zealand groper (Polyprion oxygeneios).},
  author={S. Anderson and D. Hulston and S. M. McVeagh and V. L. Webb and P. Smith},
  journal={Journal of microbiological methods},
  volume={79 1},
  • S. Anderson, D. Hulston, +2 authors P. Smith
  • Published 2009
  • Biology, Medicine
  • Journal of microbiological methods
  • An in vitro culture method was developed for the ciliated protozoa Uronema marinum isolated from New Zealand aquacultured groper (Polyprion oxygeneios). Both formulated media and sterile seawater supplemented with homogenised fish tissue as a food source supported growth of U. marinum achieving cell densities of up to 1 x 10(5)cells/mL in culture. A cryopreservation method based on a cryomix formula of 20% glycerol, 10% fetal bovine serum and 70% cultured U. marinum, incorporating a slow freeze… CONTINUE READING
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