In vitro cultivation of human renal cell cancer

@article{Williams1976InVC,
  title={In vitro cultivation of human renal cell cancer},
  author={Richard D. Williams and A. Y. Elliott and Nell Stein and Elwin E. Fraley},
  journal={In Vitro},
  year={1976},
  volume={12},
  pages={623-627}
}
A technique for initiating and propagating epithelial cell cultures of human renal cell cancer and adjacent nontumor kidney is described. Seventy-five percent of the tumors and 79% of the adjacent kidney specimens cultured with this method have shown initial outgrowth and have been subcultured at least once. Two renal cell cancer cultures initiated by this method have now been in continuous culture more than 6 months, have been subcultured 27 and 19 times, and now appear to be stable lines. The… CONTINUE READING