In vitro cultivation of Anaplasma marginale in bovine erythrocytes co-cultured with endothelial cells.

@article{Waghela1997InVC,
  title={In vitro cultivation of Anaplasma marginale in bovine erythrocytes co-cultured with endothelial cells.},
  author={Suryakant D. Waghela and D. Cruz and Robert E. Droleskey and John R. Deloach and Gale Wagner},
  journal={Veterinary parasitology},
  year={1997},
  volume={73 1-2},
  pages={
          43-52
        }
}
Primary cultures of Anaplasma marginale infected erythrocytes were used to determine conditions for in vitro cultivation of the rickettsia. The infected erythrocytes that were maintained by regular addition of Glasgow's MEM with fetal calf serum and uninfected erythrocytes showed a 1-5% increase in percent infected erythrocytes on the evaluation of Giemsa stained smears. This increase in parasitemia resulted in up to 70% change in the number of infected erythrocytes. Co-culture of the infected… CONTINUE READING