In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics

  title={In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics},
  author={Mithilesh Mishra and Jun Kashiwazaki and Tomoko Takagi and Ramanujam Srinivasan and Yinyi Huang and Mohan K Balasubramanian and Issei Mabuchi},
  journal={Nature Cell Biology},
Cytokinesis in many eukaryotes involves the contraction of an actomyosin-based contractile ring. However, the detailed mechanism of contractile ring contraction is not fully understood. Here, we establish an experimental system to study contraction of the ring to completion in vitro. We show that the contractile ring of permeabilized fission yeast cells undergoes rapid contraction in an ATP- and myosin-II-dependent manner in the absence of other cytoplasmic constituents. Surprisingly, neither… CONTINUE READING
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