In vitro colony assay for a new class of megakaryocyte precursor: colony-forming unit megakaryocyte (CFU-M).

@article{Nakeff1976InVC,
  title={In vitro colony assay for a new class of megakaryocyte precursor: colony-forming unit megakaryocyte (CFU-M).},
  author={Alexander Nakeff and S M Daniels-McQueen},
  journal={Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine},
  year={1976},
  volume={151 3},
  pages={587-90}
}
A plasma culture system has been used successfully to grow and quantitate megakaryocyte colonies from mouse bone marrow following their staining for acetylcholinesterase activity in situ. Colonies averaging about six acetylcholinesterase-positive cells appear with a peak incidence after 4 days in culture with a plating efficiency of one colony formed for every 10(4) nucleated cells plated.