In vitro chromosome doubling of nineZantedeschia cultivars

  title={In vitro chromosome doubling of nineZantedeschia cultivars},
  author={Daniel Cohen and Jia-Long Yao},
  journal={Plant Cell, Tissue and Organ Culture},
Tetraploid plants have been produced from nineZantedeschia cultivars usingin vitro colchicine treatment. Rapidly-multiplying shoot cultures were treated on a medium containing 0.05% colchicine for 1, 2 or 4 days to induce chromosome doubling. Following the treatment, most shoots were killed but the surviving shoots were multiplied for several subcultures. These shoots were then rootedin vitro and transferred to a greenhouse. Plants were screened 2 months later by measuring stomatal length, and… CONTINUE READING
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