In vitro characterization of astaxanthin biosynthetic enzymes.

  title={In vitro characterization of astaxanthin biosynthetic enzymes.},
  author={Paul D Fraser and Yuri Miura and Norihiko Misawa},
  journal={The Journal of biological chemistry},
  volume={272 10},
Escherichia coli strains expressing the marine bacteria (Agrobacterium aurantiacum and Alcaligenes sp. strain PC-1) astaxanthin biosynthetic genes (crtZ and W), Haematococcus pluvialis bkt, and Erwinia uredovora crtZ genes were used for in vitro characterization of the respective enzymes. Specific enzyme assays indicated that all of the enzymes are bifunctional, in that the CrtZ enzymes formed zeaxanthin from beta-carotene via beta-cryptoxanthin, as well as astaxanthin from canthaxanthin via… CONTINUE READING
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