In vitro changes in porcine ovarian granulosa cells induced by copper.

@article{Roychoudhury2014InVC,
  title={In vitro changes in porcine ovarian granulosa cells induced by copper.},
  author={Shubhadeep Roychoudhury and Jozef Bulla and A V Sirotkin and Adriana Koles{\'a}rov{\'a}},
  journal={Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering},
  year={2014},
  volume={49 6},
  pages={625-33}
}
Objective of this in vitro study was to examine the secretion activity (progesterone and insulin-like growth factor I) of porcine ovarian granulosa cells after copper (Cu) addition and to outline a potential intracellular mediator (cyclin B1) of its effects. It also aimed at investigating the apoptotic potential of Cu on porcine ovarian granulosa cells after addition in vitro. Ovarian granulosa cells were incubated with copper sulphate (CuSO4·5H2O) at the doses 0.33, 0.40, 0.50, 1.0 and 2.0 μL… CONTINUE READING