In vitro chamber specification during embryonic stem cell cardiogenesis. Expression of the ventricular myosin light chain-2 gene is independent of heart tube formation.

@article{MillerHance1993InVC,
  title={In vitro chamber specification during embryonic stem cell cardiogenesis. Expression of the ventricular myosin light chain-2 gene is independent of heart tube formation.},
  author={Wanda C. Miller-Hance and M Lacorbi{\`e}re and Stephen J. Fuller and Sylvia M. Evans and Gary Lyons and Christian Schmidt and Jeffrey Robbins and Kenneth R. Chien},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 33},
  pages={25244-52}
}
The molecular cues that control patterning of the heart tube during early cardiogenesis are largely unknown. The present study has explored the embryonic stem (ES) cell differentiation system to determine if this in vitro model could be useful in studying the process of regional specification of cardiac muscle cells at the earliest possible stages. As assessed by polymerase chain reaction, ribonuclease protection, in situ hybridization, and immunohistochemical analyses, ES cell differentiation… CONTINUE READING