In vitro attachment of bilins to apophycocyanin. III. Properties of the phycoerythrobilin adduct.

@article{Arciero1988InVA,
  title={In vitro attachment of bilins to apophycocyanin. III. Properties of the phycoerythrobilin adduct.},
  author={David M. Arciero and Jerry L Dallas and Alexander N. Glazer},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 34},
  pages={18358-63}
}
Addition of phycoerythrobilin (PEB) to apophycocyanin at pH 7.0 resulted in covalent adduct formation. The adduct showed absorbance maxima at 575 and 605 nm and fluorescence emission maxima at 582 and 619 nm. Analysis of bilin peptides obtained upon tryptic digestion of the adduct showed residues alpha-Cys-84 and beta-Cys-82 to be the sites of bilin addition. The product of PEB addition at the alpha-Cys-84 site was shown by 1H NMR analysis to be a dihydrobiliviolinoid peptide-linked pigment… CONTINUE READING

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