In vitro association between the helper component-proteinase of zucchini yellow mosaic virus and cuticle proteins of Myzus persicae.

@article{Dombrovsky2007InVA,
  title={In vitro association between the helper component-proteinase of zucchini yellow mosaic virus and cuticle proteins of Myzus persicae.},
  author={Aviv Dombrovsky and Natan Gollop and Songbi Chen and Nor Chejanovsky and Benjamin Raccah},
  journal={The Journal of general virology},
  year={2007},
  volume={88 Pt 5},
  pages={1602-10}
}
Potyviruses, as typical non-persistently transmitted viruses, are carried within the stylets of aphids. Cuticle proteins (CuPs), which are a major component of the insect cuticle, were examined for in vitro binding to the potyviral helper component-proteinase (HC-Pro). Proteins in 8 M urea extracts from Myzus persicae were separated by SDS-PAGE, electroblotted onto membranes and identified as CuPs by using specific antibodies to M. persicae CuP. Blotted M. persicae protein extracts were… CONTINUE READING

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