In vitro assembly of repertoires of antibody chains on the surface of phage by renaturation.

@article{Figini1994InVA,
  title={In vitro assembly of repertoires of antibody chains on the surface of phage by renaturation.},
  author={M Figini and James D Marks and Greg Winter and Andrew D. Griffiths},
  journal={Journal of molecular biology},
  year={1994},
  volume={239 1},
  pages={68-78}
}
Antibodies can be made from repertoires of associated heavy and light chains displayed on the surface of bacteriophage, and are readily diversified by random point mutation or by chain shuffling. To make extensive variations around the "core" antigen binding contacts of a crystallographically solved mouse antibody NQ10/12.5 (gamma l, kappa), the NQ10 light chain was assembled in vitro with a repertoire of about 10(7) human heavy chains displayed on the surface of phage, and selected by binding… CONTINUE READING

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