In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90.

@article{Iki2010InVA,
  title={In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90.},
  author={Taichiro Iki and Manabu Yoshikawa and Masaki Nishikiori and Mauren C Jaudal and Eiko Matsumoto-Yokoyama and Ichiro Mitsuhara and Tetsuo Meshi and Masayuki Ishikawa},
  journal={Molecular cell},
  year={2010},
  volume={39 2},
  pages={282-91}
}
RNA-induced silencing complexes (RISCs) play central roles in posttranscriptional gene silencing. In plants, the mechanism of RISC assembly has remained elusive due to the lack of cell-free systems that recapitulate the process. In this report, we demonstrate that plant AGO1 protein synthesized by in vitro translation using an extract of evacuolated tobacco protoplasts incorporates synthetic small interfering RNA (siRNA) and microRNA (miRNA) duplexes to form RISCs that sequester the single… CONTINUE READING
Highly Influential
This paper has highly influenced 15 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
78 Citations
51 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 78 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 51 references

In vitro translation and replication of tobamovirus RNA in a cell-free extract of evacuolated tobacco BY-2 protoplasts

  • K. Ishibashi, K. Komoda, M. Ishikawa
  • Biotechnology in Agriculture and Forestry, T…
  • 2006
Highly Influential
5 Excerpts

Similar Papers

Loading similar papers…