In vitro assays to characterize inhibitors of the activation of small G proteins by their guanine nucleotide exchange factors.

@article{Zeeh2008InVA,
  title={In vitro assays to characterize inhibitors of the activation of small G proteins by their guanine nucleotide exchange factors.},
  author={Jean-Christophe Zeeh and Bruno Antonny and Jacqueline Cherfils and Mahel Zeghouf},
  journal={Methods in enzymology},
  year={2008},
  volume={438},
  pages={41-56}
}
Guanine nucleotide exchange factors (GEFs) are essential regulators of the spatiotemporal conditions of small GTP-binding protein (SMG) activation. Their cellular activities combine the biochemical stimulation of GDP/GTP exchange, which leads to the active conformation of the SMG, to the detection of upstream signals and, in some cases, interaction with… CONTINUE READING