In vitro assays to analyze translocation of the model secretory preprotein alkaline phosphatase.

@article{Gouridis2010InVA,
  title={In vitro assays to analyze translocation of the model secretory preprotein alkaline phosphatase.},
  author={Giorgos Gouridis and Spyridoula Karamanou and Marina Koukaki and Anastassios Economou},
  journal={Methods in molecular biology},
  year={2010},
  volume={619},
  pages={157-72}
}
Almost one-third of the proteins synthesized in the cytosol of cells ends up in membranes or outside the cell. Secretory polypeptides are synthesized as precursor proteins that carry N-terminal signal sequences. Secretion is catalyzed by the "translocase" that comprises a channel-clamp protein and an ATPase motor. Translocase activities have been fully… CONTINUE READING