In vitro assays of orphan glycosyltransferases and their application to identify Notch xylosyltransferases.

Abstract

Here we describe a systematic approach to determine the activity of putative glycosyltransferases with a focus on orphan members of the glycosyltransferase 8 family. An assay that measures the hydrolysis activity of glycoslytransferases can indicate the donor nucleotide sugar specificity without previous knowledge about the acceptor. Knowing the donor… (More)
DOI: 10.1007/978-1-62703-465-4_23

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