In vitro appearance of large human histamine-containing basophilic cells.


The appearance of large human basophilic cells containing histamine was followed to reflect cell maturation in cultures of peripheral blood leukocytes. Aliquots were taken on day 0 and after 7, 9-10 and 14 days in culture, and the basophilic cell numbers were determined in 200 microliter cytospin specimens stained with Wright's stain. The number of small… (More)


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